Drug susceptibility testing of Mycobacterium tuberculosis by two phenotypic methods
نویسندگان
چکیده
منابع مشابه
Correlation of different phenotypic drug susceptibility testing methods for four fluoroquinolones in Mycobacterium tuberculosis.
BACKGROUND Molecular resistance testing fails to explain all fluoroquinolone resistance, with a continued need for a suitable rapid phenotypic drug susceptibility testing method. OBJECTIVE To evaluate the optimal method for phenotypic fluoroquinolone susceptibility testing. METHODS Using Löwenstein-Jensen medium, Middlebrook 7H11 agar, BACTEC-MGIT 960 and the resazurin microtitre plate assa...
متن کاملComparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing with Mycobacterium tuberculosis.
Mycobacterium tuberculosis converts pyrazinamide to its active form by using the enzyme pyrazinamidase. This enzyme is coded for on the pncA gene, and mutations in the pncA gene result in absence of active enzyme, conferring resistance to the drug pyrazinamide. We investigated 27 strains of Mycobacterium tuberculosis suspected of being multidrug resistant. Each isolate was tested for susceptibi...
متن کاملNonweekend schedule for BACTEC drug susceptibility testing of Mycobacterium tuberculosis.
Determination of the drug susceptibility of Mycobacterium tuberculosis by conventional methods using an agar-based medium may take 3 weeks or more to complete. On the other hand, results on positive cultures are generally available in 4 to 7 days with the radiometric (BACTEC, Johnston Laboratories, Towson, Md.) procedure. One disadvantage to the latter is the requirement to determine the quanti...
متن کاملNitrate reductase assay for drug susceptibility testing of Mycobacterium tuberculosis.
In their recent article, Kristian Ängeby et al. (4) reported a novel test for drug susceptibility testing of Mycobacterium tuberculosis. However, it should be clarified that this method was previously published in 1989 (3) by Emil Kalfin, (National Institute for Lung Diseases, Sofia, Bulgaria) and Andreas Engibarov (National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria). It is n...
متن کامل[External quality assessment of drug susceptibility testing for Mycobacterium tuberculosis].
OBJECTIVE The Committee for Mycobacterial Examinations has planned and implemented the third external quality assessment of drug susceptibility testing for Mycobacterium tuberculosis to the hospital and private laboratories. METHOD The Committee delivered 20 M. tuberculosis strains, exactly pairs of 10 strains, which were evaluated and standardized for the drug resistance pattern in the WHO/ ...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2012
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2012.05.968